Tian Liu
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Microbial Secondary Metabolite, Phlegmacin B-1, as a Novel Inhibitor of Insect Chitinolytic Enzymes
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Indexed by:期刊论文

Date of Publication:2017-05-17

Journal:JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY

Included Journals:SCIE、EI、PubMed

Volume:65

Issue:19

Page Number:3851-3857

ISSN No.:0021-8561

Key Words:chitinase; hexosaminidase; inhibitor; phlegmacin; microbial secondary metabolites

Abstract:Periodic chitin remodeling during insect growth and development requires a synergistic action of two glycosyl hydrolase (GH) family enzymes, GH18 chitinase and GH2O beta-N-acetylhexosaminidase (Hex). Inhibiting either or both of these enzymes is a promising strategy for pest control and management. In this study, Of Chi-h (a GH18 chitinase) and OfHexl (a GH2O Hex) from Ostrinia furnacalis were used to screen a library of microbial secondary metabolites. Phlegmacin B, was found to be the inhibitor of both Of Chi-h and OfHexl with K-i varues of 5.5 mu M and 26 mu M, respectively. Injection and feeding experiments demonstrated that phlegmacin B-1 has insecticidal effect on 0. furnacalis's larvae. Phlegmacin B-1 was predicted to bind to the active pockets of both Of Chi-h and OfHexl. Phlegmacin B, also showed moderate inhibitory activities against other bacterial and insect GH18 enzymes. This work provides an example of exploiting microbial secondary metabolites as potential pest control and management agents.

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Gender:Male

Alma Mater:Dalian University of Technology

Degree:Doctoral Degree

School/Department:School of Bioengineering

Discipline:Biochemical Engineering. Biochemistry and Molecular Biolog

Business Address:Room 445, School of Bioengineering

Contact Information:tianliu@dlut.edu.cn

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