Title of Paper:Assessment of 4-nitro-1,8-naphthalic anhydride reductase activity in homogenates of bakers' yeast by reversed-phase high-performance liquid chromatography

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Date of Publication:2007-03-01

Journal:JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES

Included Journals:SCIE、EI、PubMed

Volume:847

Issue:2

Page Number:82-87

ISSN No.:1570-0232

Key Words:nitroaryl; nitroarene; arylhydroxylamine; aminoaryl; bakers' yeast; nitroarylreductase; HPLC; TLC

Abstract:A simple reversed-phase high-performance liquid chromatographic (RP-HPLC) method was developed for the simultaneous determination of yield and conversionratio of 4-nitro-1,8-naphthalic anhydride to 4-amino-1,8-naphthalic: anhydride following incubation with a crude bakers' yeast homogenate. The analytes were separated on a C18 column in gradient mode. The detection limit of 4-amino-1,8-naphthalic anhydride is 10ng/mu l when using a 10 mu l sample injection volume. The nitroreductase activity in the homogenate system can be assessed during the bioconversion process. The method can be used for the simultaneous detection of 4-hydroxylamini)-1,8-naphthalic anhydride, an intermediate with limited stability. (c) 2006 Elsevier B.V. All rights reserved.