论文类型：期刊论文
发表刊物：BIOTECHNOLOGY LETTERS
收录刊物：PubMed、SCIE、Scopus
卷号：25
期号：2
页面范围：101-104
ISSN号：0141-5492
关键字：Escherichia coli; expression; gloshedobin; thrombin-like enzyme
摘要：Gloshedobin, a thrombin-like enzyme from the venom of Gloydius shedaoensis, was expressed in Escherichia coli using expression vector pET-32a(+). The gene was expressed under T7 promotor with a fusion partner of Thx. Tag and a 6xHis. Tag at its 5' terminal. After induction by IPTG for 6 h, the recombinant enzyme was expressed in the cytoplasm. Expression at 25degreesC gave twice the amount of recombinant gloshedobin in cytoplasm than at 37degreesC.