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论文类型： 期刊论文

发表时间： 2016-07-01

发表刊物： ACS SENSORS

收录刊物： SCIE、EI、Scopus

卷号： 1

期号： 7

页面范围： 958-962

ISSN号： 2379-3694

关键字： airborne bacteria; microfluidics; point-of-care detection; LAMP

摘要： Rapid capture and detection of airborne pathogen are essential for disease prevention and public safety. In this study, we presented a microfluidic system that could capture and enrich airborne pathogens as well as performing high-throughput LAMP analysis. The system was validated by five species of bacteria and showed good stability and specificity. Detection limit down to approximately 24 cells per reaction was achieved for Staphylococcus aureus, and without the process of DNA purification. To the best of our knowledge, this is the first report to describe a microfluidic system for airborne pathogen capture and high-throughput LAMP analysis. The results could be detected by the naked eye, suggesting that the system could have great potential application in clinical diagnostics and point-of-care detection.