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论文类型： 期刊论文

发表时间： 2016-06-01

发表刊物： ACTA MECHANICA SINICA

收录刊物： SCIE、EI、ISTIC、CSCD、Scopus

卷号： 32

期号： 3

页面范围： 422-429

ISSN号： 0567-7718

关键字： Single cell trapping; Microfluidics; Stagnation point flow; Physical barrier; Hydrodynamic tweezers; Dynamic biochemical signal

摘要： Single cell trapping in vitro by microfluidic device is an emerging approach for the study of the relationship between single cells and their dynamic biochemical microenvironments. In this paper, a hydrodynamic-based microfluidic device for single cell trapping is designed using a combination of stagnation point flow and physical barrier. The microfluidic device overcomes the weakness of the traditional ones, which have been only based upon either stagnation point flows or physical barriers, and can conveniently load dynamic biochemical signals to the trapped cell. In addition, it can connect with a programmable syringe pump and a microscope to constitute an integrated experimental system. It is experimentally verified that the microfluidic system can trap single cells in vitro even under flow disturbance and conveniently load biochemical signals to the trapped cell. The designed micro-device would provide a simple yet effective experimental platform for further study of the interactions between single cells and their microenvironments.