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发布时间：2019-03-11

论文类型：期刊论文

发表时间：2017-09-07

发表刊物：ANALYTICAL METHODS

收录刊物：EI、SCIE、Scopus

卷号：9

期号：33

页面范围：4858-4862

ISSN号：1759-9660

摘要：Ultrafiltration combined with liquid chromatography-mass spectrometry (UF-LC-MS) is an efficient affinitybased method to screen potential ligands of biomacromolecules from natural sources. In order to investigate the effects of constituents from Pueraria labta Ohwi on tyrosinase, a UF-LC-MS method for screening tyrosinase ligands was established. Seven constituents were screened as tyrosinase ligands with high affinity and their structures were elucidated as isoflavones and isoflavone glycosides by mass spectrum analysis, among which puerarin exhibited much stronger inhibitory activity in vitro than arbutin against tyrosinase. An in silico docking study indicates that the interaction between puerarin and tyrosinase mainly derived from hydrogen bonds and pi-cation interaction.