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论文类型： 期刊论文

发表时间： 2014-08-01

发表刊物： MOLECULAR BIOLOGY REPORTS

收录刊物： SCIE、PubMed

卷号： 41

期号： 8

页面范围： 5385-5394

ISSN号： 0301-4851

关键字： Tomato; miRNA; Target gene; miR398; Stress response

摘要： MicroRNAs are similar to 22 nt non-coding endogenous RNAs which play important regulation roles in various species. By using homology-based computational research, 14 novel and conserved tomato miRNAs belonging to ten families were identified from EST, GSS, and nucleotide sequences. Real-time PCR analysis of these miRNAs demonstrated their expression in tomato afterwards. Meanwhile, a total of 36 potential targets were predicted for the ten miRNAs using psRNATarget. The target genes were mainly involved in metabolism, transmembrane transport, stress response, and transcription regulation. According to our experiment, miR398 was down-regulated on different levels under biotic and abiotic stresses, suggesting that miR398 might be involved in tomato stress regulatory network. Our results supplement the findings of tomato miRNAs and also suggest crucial regulatory functions of miRNAs in stress responses.