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论文类型：期刊论文

发表时间：2021-01-10

发表刊物：ANALYST

卷号：145

期号：20

页面范围：6572-6578

ISSN号：0003-2654

摘要：Ag(+)plays an important role in DNA mismatch technology due to its affinity for cytosine in DNA. This article introduces a strategy to control the enzyme digesting reaction by utilizing the characteristics of C-Ag+-C mismatches, effectively regulating and controlling the activity of the E6 DNAzymeviachanging the structure of its conservative domain. We designed a series of basic logic gates, a "Yes" Gate, an "Or" Gate and an "Inhibit" Gate. Cysteine (Cys) can combine with Ag+, reducing the concentration of Ag(+)in solution, thus restraining the C-Ag+-C mismatch effect. Based on this principle, we regard Cys as a threshold, and designed a type of "Inhibit" Gate based on input quantity by changing the concentration of Ag+, thus generating different statues of logic output. On this basis, the E6 DNAzyme and Ag10c DNAzyme can be integrated into new systems with the function of logic operation circuit based on the control of Ag(+)concentration in solution. This system could represent three different states of logical expression by controlling the quantity of Ag(+)and Cys.