Hits:

Indexed by:期刊论文

Date of Publication:2017-05-01

Journal:BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY

Included Journals:SCIE、EI、PubMed、Scopus

Volume:64

Issue:3

Page Number:400-405

ISSN No.:0885-4513

Key Words:encapsulation; engineering islets; islets isolation; islet cell clusters; reaggregation

Abstract:After islet isolation, diffusion has become the main mechanism to transport oxygen and nutrients into the core of islets. However, diffusion has limitations, by which nutrients cannot effectively reach the core of large islets and can eventually cause core cell death and islet loss. This problem can be resolved by dispersing islets into single islet cells, but single islet cells do not exhibit insulin release function in in vitro culture. In this study, we intended to establish a new islet engineering approach by forming islet cell clusters to improve islet survival and function. Therefore, alginate gels were used to encapsulate islet cells to form artificial islets after dispersion of islets into single cells. The shape of the islet cell clusters was similar to native islets, and the size of the islet cell clusters was limited to a maximum diameter of 100 mu m. By limiting the diameter of this engineered islet cell cluster, cell viability was nearly 100%, a significant improvement over natural islets. Importantly, islet cell clusters express the genes of islets, including Isl-1, Gcg, and insulin-1, and insulin secretion ability was maintained in vitro. C (C) 2016 International Union of Biochemistry and Molecular Biology, Inc.