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Indexed by:期刊论文

Date of Publication:2022-06-29

Journal:过程工程学报

Issue:6

Page Number:964-968

ISSN No.:1009-606X

Abstract:Alginate (ALG) microspheres containing bovine serum albumin (BSA) were prepared by an emulsification technique. Soybean salad oil used widely in food industry was chosen as an oil phase. Orthogonal experimental design was used to find the optimal preparation conditions by comparing the encapsulation efficiency of BSA, loading capability and average diameter of microspheres. The fine spherical alginate microspheres with an average diameter of 10 μn were obtained under the appropriate conditions. The encapsulation efficiency of BSA and the BSA loading capability were 70% and 4% (ω), respectively. As the concentration of ALG increased and the amount of BSA decreased, the encapsulation efficiency of BSA increased, however, BSA loading capability decreased. The in vitro release profile showed a fast release rate of encapsulated BSA, particularly in phosphate buffered saline solution, owing to the excellent pH responsive properties of alginate microspheres. Finally, electrophoresis experiment illustrated that the process of encapsulation had no effect on the molecular weight of the entrapped protein.

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