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个人信息Personal Information
教授
博士生导师
硕士生导师
主要任职：Director, State Key laboratory of Fine Chemicals
其他任职：精细化工国家重点实验室主任、国务院学科评议组成员
性别：男
毕业院校：大连理工大学
学位：博士
所在单位：化工学院
学科：应用化学. 精细化工. 化学生物学
办公地点：大连理工大学精细化工国家重点实验室
西部校区化工实验楼F-202#
http://peng-group.dlut.edu.cn/
联系方式：大连理工大学精细化工国家重点实验室 西部校区化工实验楼F-202 辽宁省大连市高新区凌工路2号，大连116024 Tel: 0411-84986306; Fax: 0411-84986292；课题组网址：http://peng-group.dlut.edu.cn/
电子邮箱：pengxj@dlut.edu.cn
Fluorescence imaging of SO2 derivatives in Daphnia magna with a mitochondria-targeted two-photon ratiometric fluorescent probe
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论文类型：期刊论文
发表时间：2018-01-01
发表刊物：SENSORS AND ACTUATORS B-CHEMICAL
收录刊物：SCIE、EI
卷号：254
页面范围：709-718
ISSN号：0925-4005
关键字：Ratiometric fluorescent probe; Two-photon; Mitochondria-targeted; Bioimaging
摘要：Sulfur dioxide (SO2), one of the reactive sulfur species (RSS), plays significant roles in various physiology and pathological processes. Excessive inhalation of SO2 will be conducive to the occurrence of respiratory diseases, neurological disorders and cardiovascular diseases, even lung cancer. Hence, it's vital to develop efficient methods for real-time monitoring of SO2 in vivo. In this work, a two-photon ratiometric fluorescent probe TP-SO2 was proposed based on Michael addition mechanism, which showed a 143-fold enhancement of fluorescence intensity ratio (F483 JF600,) toward SO2 with outstanding sensitivity (DL 0.161 M), rapid response (35s) and high selectivity. Probe TP-SO2 showed good cell membrane permeability and localized in mitochondria. More importantly, to the best of our knowledge, it is the first time that the probe TP-SO2 was used for visualizing exogenous SO2 derivative changes in Daphnia magna through two-photon fluorescence microscopy, which is of great importance for biological research. (C) 2017 Elsevier B.V. All rights reserved.