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个人信息Personal Information
教授
博士生导师
硕士生导师
主要任职：Director, State Key laboratory of Fine Chemicals
其他任职：精细化工国家重点实验室主任、国务院学科评议组成员
性别：男
毕业院校：大连理工大学
学位：博士
所在单位：化工学院
学科：应用化学. 精细化工. 化学生物学
办公地点：大连理工大学精细化工国家重点实验室
西部校区化工实验楼F-202#
http://peng-group.dlut.edu.cn/
联系方式：大连理工大学精细化工国家重点实验室 西部校区化工实验楼F-202 辽宁省大连市高新区凌工路2号，大连116024 Tel: 0411-84986306; Fax: 0411-84986292；课题组网址：http://peng-group.dlut.edu.cn/
电子邮箱：pengxj@dlut.edu.cn
A novel "turn-on" fluorescent probe with a large stokes shift for homocysteine and cysteine: Performance in living cells and zebrafish
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论文类型：期刊论文
发表时间：2018-04-15
发表刊物：SENSORS AND ACTUATORS B-CHEMICAL
收录刊物：SCIE、EI
卷号：259
页面范围：233-240
ISSN号：0925-4005
关键字：Thiol probe; "Turn-on" fluorescence; Large stokes shift; Cell imaging; Zebrafish imaging
摘要：Carbazole-based fluorescent probes have a significant edge on structure and optical properties, but stokes shift of them were mostly less than 100nm. In this work, a novel carbazole-based fluorescent probe, which featured with a large stokes shift (128nm), was used to effectively detect homocysteine (Hcy) and cysteine (Cys). This probe revealed obvious color variation and a turn-on alteration of fluoresence toward Hcy and Cys. Furthermore, the probe works under physiological conditions and can image thiol in living cells as well as zebrafish because of its wonderful cell permeability. Notably, it provides a possibility to detect endogenous bio-thiols quantitatively in living cells. (c) 2017 Elsevier B.V. All rights reserved.