彭孝军

个人信息Personal Information

教授

博士生导师

硕士生导师

主要任职:Director, State Key laboratory of Fine Chemicals

其他任职:精细化工国家重点实验室主任、国务院学科评议组成员

性别:男

毕业院校:大连理工大学

学位:博士

所在单位:化工学院

学科:应用化学. 精细化工. 化学生物学

办公地点:大连理工大学精细化工国家重点实验室
西部校区化工实验楼F-202#  
http://peng-group.dlut.edu.cn/

联系方式:大连理工大学精细化工国家重点实验室 西部校区化工实验楼F-202 辽宁省大连市高新区凌工路2号,大连116024 Tel: 0411-84986306; Fax: 0411-84986292;课题组网址:http://peng-group.dlut.edu.cn/

电子邮箱:pengxj@dlut.edu.cn

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A alpha-KA fluorescent probe for discrimination of blood cancer serum

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论文类型:期刊论文

发表时间:2017-10-01

发表刊物:CHINESE CHEMICAL LETTERS

收录刊物:SCIE、Scopus

卷号:28

期号:10,SI

页面范围:1991-1993

ISSN号:1001-8417

关键字:Biomarker; Fluorescent probe; alpha-Ketoglutaricacid; Actue myeloid leukemia (AML); Serum detection

摘要:alpha-Ketoglutaric acid (alpha-KA) is an important metabolic intermediate in tricarboxylic acid circle in our body. The mutations of isocitrate dehydrogenase-1 (IDH1) and isocitrate dehydrogenase-2 (IDH2), however, will lead to the transformation of alpha-KA into 2-hydroxyglutarate (2-HG), which is confirmed to closely related to actue myeloid leukemia (AML). Therefore it is of great significance to detect alpha-KA level changes in serum. In this paper, a fluorescent "off-on" probe CH for alpha-KA was designed based on naphthalimide fluorophore by introducing a hydrazine group for alpha-KA recognition and a long alkyl amino chain to enhance PET efficiency and water solubility. Cetyltrimethyl ammonium bromide (CTAB) was added toform self-assembly micelles for accelerating the recognition process. CH shows a 28-fold fluorescence enhancement ((I- I-0)/I-0 at 550 nm) over other biological species by optimizing the chemical recognition process of CH with alpha-KA. Significantly, CH was successfully applied for thefluorescence discrimination of all kinds of blood cancer serum samples. This work would provide a potential method that is quick and convenient for sensing alpha-KA and may promote fluorescence detection in clinical diagnosis. (C) 2017 Chinese Chemical Society and Institute of Materia Medica, Chinese Academy of Medical Sciences. Published by Elsevier B.V. All rights reserved.