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个人信息Personal Information
教授
博士生导师
硕士生导师
主要任职：Director, State Key laboratory of Fine Chemicals
其他任职：精细化工国家重点实验室主任、国务院学科评议组成员
性别：男
毕业院校：大连理工大学
学位：博士
所在单位：化工学院
学科：应用化学. 精细化工. 化学生物学
办公地点：大连理工大学精细化工国家重点实验室
西部校区化工实验楼F-202#
http://peng-group.dlut.edu.cn/
联系方式：大连理工大学精细化工国家重点实验室 西部校区化工实验楼F-202 辽宁省大连市高新区凌工路2号，大连116024 Tel: 0411-84986306; Fax: 0411-84986292；课题组网址：http://peng-group.dlut.edu.cn/
电子邮箱：pengxj@dlut.edu.cn
A fluorescent and colorimetric probe for imaging the mitochondrial sulfur dioxide in living cells
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论文类型：期刊论文
发表时间：2018-11-10
发表刊物：SENSORS AND ACTUATORS B-CHEMICAL
收录刊物：SCIE、Scopus
卷号：273
页面范围：899-905
ISSN号：0925-4005
关键字：Mitochondria-targeted; Colorimetric; Imaging; Fluorescence; Environment detection; Sulfur dioxide
摘要：Sulfur dioxide (SO2), one of active sulfur species (ASS), plays important roles in various physiologies and pathological processes. Hence, it is urgent to monitor the fluctuation of mitochondrial SO2 in vivo. In this work, a novel mitochondria-targeting probe CZ was designed and synthesized based on carbazole derivative, which showed that probe was employed to the detection of SO2 with high selectivity, rapid response as well as naked eyes. Besides, an excellent linear relationship (R-2 = 0.9977) was observed and the detection limit was calculated as low as 0.50411M (3 sigma/k). Surprisingly, with addition of SO2 derivative, fluorescence intensity ratios (F-475 nm/F-607 nm) of probe CZ increased by 1104-fold ranging from 0.007 to 7.73. Owing to its biocompatibility, probe CZ was also successfully employed for monitoring of mitochondrial SO2 by fluorescence confocal microscopy.