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个人信息Personal Information
教授
博士生导师
硕士生导师
主要任职：Director, State Key laboratory of Fine Chemicals
其他任职：精细化工国家重点实验室主任、国务院学科评议组成员
性别：男
毕业院校：大连理工大学
学位：博士
所在单位：化工学院
学科：应用化学. 精细化工. 化学生物学
办公地点：大连理工大学精细化工国家重点实验室
西部校区化工实验楼F-202#
http://peng-group.dlut.edu.cn/
联系方式：大连理工大学精细化工国家重点实验室 西部校区化工实验楼F-202 辽宁省大连市高新区凌工路2号，大连116024 Tel: 0411-84986306; Fax: 0411-84986292；课题组网址：http://peng-group.dlut.edu.cn/
电子邮箱：pengxj@dlut.edu.cn
A Novel AIE Plus ESIPT Fluorescent Probe with a Large Stokes Shift for Cysteine and Homocysteine: Application in Cell Imaging and Portable Kit
点击次数：
论文类型：期刊论文
发表时间：2018-11-07
发表刊物：INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH
收录刊物：SCIE、Scopus
卷号：57
期号：44
页面范围：15216-15223
ISSN号：0888-5885
关键字：Amino acids; Excited states; Fluorescence; Fluorescence spectroscopy; High performance liquid chromatography; Thin layer chromatography; Transmission electron microscopy, Aggregation-induced emissions; Excited-state intramolecular proton transfer; Fluorescent probes; Low cytotoxicities; On-site inspection; Photo-stability; Response mechanisms; Salicylaldehyde, Probes
摘要：The application of fluorescent probes is limited due to the small Stokes shifts and aggregation-caused quenching (ACQ) effect when accumulated in cells. Herein, a novel colorimetric and turn-on fluorescent probe based on salicylaldehyde azine with both aggregation-induced emission (AIE) and excited-state intramolecular proton transfer (ESIPT) properties for Cys/Hcy is proposed to solve these issues. This probe showed a large Stokes shift (148 nm), low cytotoxicity as well as outstanding photostability upon recognition and the response mechanism was confirmed by fluorescence spectroscopy, High performance liquid chromatography (HPLC), thin layer chromotography (TLC), and transmission electron microscope (TEM). In addition to being used for cell imaging, a simple and user-friendly portable kit based on this probe was proposed as a new tool for the on-site inspection of more than ten microsamples simultaneously, which could effectively prevent the occurrence of false positives and visual errors.