个人信息Personal Information
教授
博士生导师
硕士生导师
主要任职:Director, State Key laboratory of Fine Chemicals
其他任职:精细化工国家重点实验室主任、国务院学科评议组成员
性别:男
毕业院校:大连理工大学
学位:博士
所在单位:化工学院
学科:应用化学. 精细化工. 化学生物学
办公地点:大连理工大学精细化工国家重点实验室
西部校区化工实验楼F-202#
http://peng-group.dlut.edu.cn/
联系方式:大连理工大学精细化工国家重点实验室 西部校区化工实验楼F-202 辽宁省大连市高新区凌工路2号,大连116024 Tel: 0411-84986306; Fax: 0411-84986292;课题组网址:http://peng-group.dlut.edu.cn/
电子邮箱:pengxj@dlut.edu.cn
Development of a red-light emission hypoxia-sensitive two-photon fluorescent probe for in vivo nitroreductase imaging
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论文类型:期刊论文
发表时间:2019-01-21
发表刊物:JOURNAL OF MATERIALS CHEMISTRY B
收录刊物:SCIE、Scopus
卷号:7
期号:3
页面范围:408-414
ISSN号:2050-750X
关键字:Mammals; Photons; Probes; Tissue, Deep tissue imaging; Emission wavelength; Fluorescence signals; Fluorescent probes; High selectivity; Red fluorescence; Red-light emission; Tissue penetrations, Fluorescence
摘要:The overexpression of nitroreductase (NTR) in hypoxia has been recognized as a biomarker of highly aggressive disease, and the development of a hypoxia-sensitive two-photon (TP) bioimaging probe with both excitation and emission wavelengths in the red-light region provides favorable deep-tissue imaging with a low background fluorescence signal. Although quite a few TP hypoxia-sensitive fluorescent probes have been reported for NTR detection, their short emission wavelength (o550 nm) limits their application. Herein, we report a red light emissive TP hypoxia-sensitive turn-on probe (NRP) by employing Nile Red as a red-emitting fluorophore and p-nitrobenzene as an NTR recognition group with improved sensitivity. The NRP probe showed obvious strong red-fluorescence enhancement in the presence of NTR and high selectivity toward NTR in aqueous solution. Our in vitro experimental results illustrated that the NRP loaded tumor cells treated under hypoxia display remarkably strong fluorescence in both OP and TP microscopy at 655 nm with 45-fold enhancement, which affords deep-tissue penetration ability. The NRP probe was also successfully applied for imaging NTR in liver tissue slices and a 4T1-bearing mice model, which is important for bioimaging applications.