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个人信息Personal Information
教授
博士生导师
硕士生导师
主要任职：Director, State Key laboratory of Fine Chemicals
其他任职：精细化工国家重点实验室主任、国务院学科评议组成员
性别：男
毕业院校：大连理工大学
学位：博士
所在单位：化工学院
学科：应用化学. 精细化工. 化学生物学
办公地点：大连理工大学精细化工国家重点实验室
西部校区化工实验楼F-202#
http://peng-group.dlut.edu.cn/
联系方式：大连理工大学精细化工国家重点实验室 西部校区化工实验楼F-202 辽宁省大连市高新区凌工路2号，大连116024 Tel: 0411-84986306; Fax: 0411-84986292；课题组网址：http://peng-group.dlut.edu.cn/
电子邮箱：pengxj@dlut.edu.cn
A NIR fluorescent sensor with large Stokes shift for the real-time visualization of endogenous hydrogen peroxide in living cells
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论文类型：期刊论文
发表时间：2019-02-01
发表刊物：JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY A-CHEMISTRY
收录刊物：SCIE、Scopus
卷号：370
页面范围：12-17
ISSN号：1010-6030
关键字：NIR emission; Hydrogen peroxide; Large Stokes shift; Cell imaging
摘要：Hydrogen peroxide (11202), regarded as the byproduct of aerobic metabolism via the electron transport chain, plays a significant role in a vast array of physiological and pathological process in the organisms. Therefore, it is of great importance to develop efficient analytical approach for monitoring the H2O2 level in living systems. Herein, in this work, a NIR fluorescent probe named NDCM-HP was developed for the highly selective detection of H2O2 by introducing boronate group as the recognition unit into the 2-(3-(4-hydroxystyryl)-5,5-dimethylcyclohex-2-enylidene) malononitrile (NDCM-OH) fluorophore which is featured by strong NIR emission, large Stokes shift, and good photostability. As a matter of fact, NDCM-HP demonstrated excellent sensing performance toward H2O2 in solution samples such as high sensitivity with about 127-fold emission enhancement, low detection limit of 70.189 nM, and good selectivity over biologically related species. Furthermore, it could be employed for detecting endogenous H2O2 fluctuation in living cells induced by different stimulators such as Phorbol myristate acetate (PMA), N-Acetylcysteine (NAC), indicating its great potential in biological application.