个人信息Personal Information
教授
博士生导师
硕士生导师
主要任职:Director, State Key laboratory of Fine Chemicals
其他任职:精细化工国家重点实验室主任、国务院学科评议组成员
性别:男
毕业院校:大连理工大学
学位:博士
所在单位:化工学院
学科:应用化学. 精细化工. 化学生物学
办公地点:大连理工大学精细化工国家重点实验室
西部校区化工实验楼F-202#
http://peng-group.dlut.edu.cn/
联系方式:大连理工大学精细化工国家重点实验室 西部校区化工实验楼F-202 辽宁省大连市高新区凌工路2号,大连116024 Tel: 0411-84986306; Fax: 0411-84986292;课题组网址:http://peng-group.dlut.edu.cn/
电子邮箱:pengxj@dlut.edu.cn
Development of a two-photon carbazole derivative probe for fluorescent visualization of G-quadruplex DNA in cells
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论文类型:期刊论文
发表时间:2019-12-01
发表刊物:DYES AND PIGMENTS
收录刊物:EI、SCIE
卷号:171
ISSN号:0143-7208
关键字:Two-photon; Carbazole derivative; Fluorescent probe; G-quadruplex DNA; Pyridostatin
摘要:G-quadruplex (G4) sequences are considered to play important roles in gene regulation, therefore the development of selective and sensitive probes for G4 DNAs is important for studying the functions of G-rich gene sequences, as well as designing of novel and effective anticancer drugs. Herein, a carbazole derivative (CZ-BT) was synthesized and characterized by a simple process for G4 DNA detection. CZ-BT was preferentially bound with G4 DNA compared with other types of nucleic acids according to the fluorescence assays. The fluorescence intensity and fluorescence lifetime of CZ-BT significantly increased after the interaction with G4 DNA. Molecular docking calculation proved the pi-pi stacking binding mode between CZ-BT and G4 DNA. Furthermore, the specificity of CZ-BT on G4 DNA was well demonstrated with the contrast of pyridostatin (PDS, a classical G-quadruplex ligand) using two-photon confocal fluorescent imaging technique and cell cycle experiment in cells. We believe that this study would give some favorable factors on developing of highly effective fluorescent probes for G4 DNA applications.