个人信息Personal Information
教授
博士生导师
硕士生导师
主要任职:Director, State Key laboratory of Fine Chemicals
其他任职:精细化工国家重点实验室主任、国务院学科评议组成员
性别:男
毕业院校:大连理工大学
学位:博士
所在单位:化工学院
学科:应用化学. 精细化工. 化学生物学
办公地点:大连理工大学精细化工国家重点实验室
西部校区化工实验楼F-202#
http://peng-group.dlut.edu.cn/
联系方式:大连理工大学精细化工国家重点实验室 西部校区化工实验楼F-202 辽宁省大连市高新区凌工路2号,大连116024 Tel: 0411-84986306; Fax: 0411-84986292;课题组网址:http://peng-group.dlut.edu.cn/
电子邮箱:pengxj@dlut.edu.cn
Rationally modifying the dicyanoisophorone fluorophore for sensing cysteine in living cells and mice
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论文类型:期刊论文
发表时间:2021-04-12
发表刊物:SENSORS AND ACTUATORS B-CHEMICAL
卷号:321
关键字:Cys detection; Regulation of pKa; Rational design of fluorophores; Fluorescent probes; In vivo imaging
摘要:Cysteine (Cys), as one of the biothiols in organisms, is regarded as a significant participant in the physiological and pathological process. However, monitoring endogenous Cys in living organisms is still hindered due to the lack of effective fluorescent probe with excellent photophysical properties. Therefore, in this work, we attempted to develop excellent fluorescent probes for Cys detection with the aim of improving the photophysical properties of fluorophores. Firstly, through the rational structural modification of the dicyanoisophorone fluorophore by introducing different electron-withdrawing or electron-donating substituent groups, we found that three fluorophores with electron-withdrawing substituent group (-F,-Cl,-Br) exhibited remarkably decreased pKa value and complete deprotonation behavior in physiological pH, endowing the fluorophores with strong fluorescence emission in physiological condition and the wide pH adaptability. Subsequently, we developed three fluorescent probes (F-Cys, Cl-Cys, Br-Cys) for the Cys detection by introducing the acrylate group as the recognition unit into these three fluorophores, respectively. By comprehensively evaluating the sensing performance of three probes toward Cys, we screened out the best fluorescent probe Br-Cys. In solution, Br-Cys exhibited high sensitivity (detection limit: 86.9 nM), fast response (10 min), large fluorescence enhancement (214-fold), good selectivity, and wide pH adaptability. Furtherly, Br-Cys was successfully employed for the specific monitoring of Cys in cells and mice, providing a potential tool for understanding the physiological and pathological functions of Cys in living systems.