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个人信息Personal Information
教授
博士生导师
硕士生导师
主要任职：Director, State Key laboratory of Fine Chemicals
其他任职：精细化工国家重点实验室主任、国务院学科评议组成员
性别：男
毕业院校：大连理工大学
学位：博士
所在单位：化工学院
学科：应用化学. 精细化工. 化学生物学
办公地点：大连理工大学精细化工国家重点实验室
西部校区化工实验楼F-202#
http://peng-group.dlut.edu.cn/
联系方式：大连理工大学精细化工国家重点实验室 西部校区化工实验楼F-202 辽宁省大连市高新区凌工路2号，大连116024 Tel: 0411-84986306; Fax: 0411-84986292；课题组网址：http://peng-group.dlut.edu.cn/
电子邮箱：pengxj@dlut.edu.cn
Immobilization of the engineered Raoultella ornithinolytica BF60 for biocatalytic synthesis of 2, 5-furandicarboxylic acid
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论文类型：期刊论文
发表时间：2021-04-12
发表刊物：Journal of microbiology and biotechnology
ISSN号：1738-8872
关键字：2, 5-furandicarboxylic acid,5-hydroxymethylfurfural,Immobilized cells,Raoultella ornithinolytica BF60
摘要：As a bio-based platform chemical, 2, 5-furandicarboxylic acid (FDCA) is a promising substitute of terephthalic acid to synthesize poly (ethylene furanoate) used for bio-substitute of polyethylene terephthalate (PET). In previous work, we engineered a Raoultella ornithinolytica BF60 strain and achieved the effective biocatalytic synthesis of FDCA from 5-hydroxymethylfurfural (HMF). Herein, to improve the reusability of the engineered R. ornithinolytica BF60 cells, we used two materials PVA1799-boric acid and sodium alginate-Ca2+ to immobilize the cells. The sodium alginate-Ca2+ was more suitable for FDCA production, and 22 mmol/L FDCA was obtained with Ca(H2PO4)2 as the cross-linker. In addition, the immobilization conditions were optimized and FDCA production was further increased to 74.8 mmol/L. Here the developed immobilization strategy for R. ornithinolytica BF60 cells may be helpful for the economical production of FDCA in the future.