|
个人信息Personal Information
教授
博士生导师
硕士生导师
主要任职：中国化学会创始会士、常务理事，中国化工学会会士
性别：男
毕业院校：大连理工大学
学位：博士
所在单位：化工学院
学科：应用化学. 精细化工. 化学生物学
办公地点：大连理工大学西部校区知顺楼F-202#
http://peng-group.dlut.edu.cn/
联系方式：大连理工大学西部校区知顺楼F-202 辽宁省大连市高新区凌工路2号，大连116024 课题组网址：http://peng-group.dlut.edu.cn/ E-mail: pengxj@dlut.edu.cn
电子邮箱：pengxj@dlut.edu.cn
Recognition of HClO in Live Cells with Separate Signals Using a Ratiometric Fluorescent Sensor with Fast Response
点击次数：
论文类型：期刊论文
发表时间：2015-09-16
发表刊物：INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH
收录刊物：SCIE、EI、Scopus
卷号：54
期号：36
页面范围：8842-8846
ISSN号：0888-5885
摘要：Hypochlorous acid (HClO), one of the reactive oxygen species (ROS), is a key microbicidal agent which is used for natural defense; however, it is also involved in several human diseases. Although several efforts have been made to develop HClO-selective fluorescent sensors, most of them display delayed response time and the ratiometric sensors are rare. In the current work, we used a 3-pyrrole BODIPY dye as a new ratiometric fluorescent sensor (BRClO) for HClO. BRClO exhibited a 177-fold fluorescence ratio (F-505 nm/F-585 nm) enhancement in the presence of HClO over other ROS with fast response time (completed within 1 s). Confocal fluorescence imaging with BRClO showed separated signals in the presence of exogenous HClO in live cells. In addition, BRClO could be easily prepared as HClO test strips to detect HClO in aqueous solution and natural tap water.