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论文类型：期刊论文

发表时间：2015-09-12

发表刊物：JOURNAL OF APPLIED POLYMER SCIENCE

收录刊物：SCIE、EI、Scopus

卷号：132

期号：35

ISSN号：0021-8995

关键字：biomaterials; drug delivery systems; micelles; self-assembly; stimuli-sensitive polymers

摘要：pH-responsiveness is highly desirable in the stimuli-responsive controlled release because of the distinct advantages of the fast response of pH-triggered release and the available pH-difference between intra-and extra-cells. The present work reported a kind of novel pH-responsive polymeric micelles, which was derived from biopolymer of 6-O-dodecyl-chitosan carbamate (DCC) and evaluated as gene-controlled release vector. The amphiphilic and amino-rich DDC was synthesized through a protection-graft-deprotection method. C-13 CP/MAS NMR, FTIR, and elemental analysis identified that dodecyls were chemoselectively grafting at 6-hydroxyls of chitosan via the pH-responsive bonds of carbamate, and the substitute degree (SD) was 14%. Transmission electron microscopy (TEM) and dynamic light scattering (DLS) showed that DCC self-assembled into polymeric micelles in aqueous solutions. The DCC polymeric micelles formed complexes with pDNA, which was elucidated by Gel retardation, TEM, and DLS. Transfection and cytotoxicity assays in A549 cells showed that DCC polymeric micelles were suitable for gene delivery. The improved transfection was attributed to the pH-responsiveness and the moderate pDNA-binding affinity, which led to easier release of pDNA intra-cells. The synthesized DCC polymeric micelles might be a promising and safe candidate as nonviral vectors for gene delivery. (C) 2015 Wiley Periodicals, Inc.