Title of Paper:Study on the extraction and back extraction of Bovine Serum Albumin using reversed micelles

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Date of Publication:2007-12-01

Journal:SEPARATION SCIENCE AND TECHNOLOGY

Included Journals:SCIE、EI、Scopus

Volume:42

Issue:16

Page Number:3741-3757

ISSN No.:0149-6395

Key Words:BSA; revered micelles; extraction ratio; selectivity

Abstract:The extraction and back extraction of Bovine Serum Albumin (BSA) using a new reversed micelle system of CTAB/isooctane/1-pentanol have been investigated. Under the optimal operating conditions in this study, the extraction ratio of BSA reaches as high as 98%. The back extraction ratio can reach 80% as pH in stripping phase approaches to the isoelectric point (pI) of BSA. UV-spectra indicated that there was no significant change in the structure of BSA enriched in stripping phase. Furthermore, the reversed micelle system was repeatedly used to extract BSA from a mixed solution of protein.