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产脂肪酶嗜碱细菌的筛选及酶学性质研究

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Indexed by:期刊论文

Date of Publication:2010-12-15

Journal:生物技术

Included Journals:PKU、CSCD

Volume:20

Issue:6

Page Number:57-61

ISSN No.:1004-311X

Key Words:脂肪酶;假单胞菌;嗜碱细菌;发酵

Abstract:目的:筛选产脂肪酶嗜碱细菌,并研究其酶学性质.方法:以豆油为唯一碳源的固体平板筛选产酶菌株,16S rDNA同源性分析确定微生物菌属,单因素实验优化产酶条件、研究酶学性质.结果:筛选出1株产脂肪酶的嗜碱菌株,鉴定为假单胞菌(Pseudomonas),命名为Pseudomonas sp.C-36.菌株产酶的最佳培养条件为:甘油2%(V/V).蛋白胨0.7%(W/V),酵母提取物0.5%(W/V),K2HPO4 0.2%(W/V),MsS04·7H2O 0.05%(W/V),NaCl 0.3%(W/V),Triton X-100 0.01%(W/V),pH 9.5,转速180r/min,37℃下培养24h,产酶量为2.782 IU/ml.该酶的最适温度和pH分别为40℃和9.0,50℃时酶活半衰期为2h.Ca2+等金属离子对该酶酶活具有促进作用,而Zn2+对酶活的抑制作用明显.有机溶剂的耐受性实验表明,该酶在疏水性有机溶剂中稳定性良好.结论:筛选得到1株嗜碱的脂肪酶产生菌C-36,并鉴定为Pseudomonas.

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