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Indexed by:期刊论文

Date of Publication:2015-07-07

Journal:ANALYST

Included Journals:SCIE、PubMed、Scopus

Volume:140

Issue:13

Page Number:4594-4598

ISSN No.:0003-2654

Abstract:Hypochlorous acid (HClO)/hypochlorite (ClO-), one of the reactive oxygen species (ROS), is a key microbicidal agent used for natural defense; however, HClO is also responsible for some human diseases. Although much effort has been made to develop HClO-selective fluorescent probes, many of them display a delayed response time and nanomole-sensitive probes are rare. In this study, we designed and synthesized an aza-coumarin based fluorescent probe AC-ClO for ClO- determination with fast response (completed within 2 min) and high sensitivity (detection limit is 25 nM). AC-ClO displayed a color change from pink to light yellow and a remarkable "turn-on" fluorescence response towards ClO-. Confocal fluorescence microscopy experiments demonstrated that the probe could be applied for the live-cell imaging of exogenous and endogenous ClO-.