Release Time:2019-03-09  Hits:

Indexed by: Journal Article

Date of Publication: 2014-04-14

Journal: CHEMISTRY-A EUROPEAN JOURNAL

Included Journals: Scopus、PubMed、EI、SCIE

Volume: 20

Issue: 16

Page Number: 4691-4696

ISSN: 0947-6539

Key Words: dyes; fluorescent probes; imaging agents; steric hindrance; viscosity

Abstract: Cellular viscosity is a critical factor in governing diffusion-mediated cellular processes and is linked to a number of diseases and pathologies. Fluorescent molecular rotors (FMRs) have recently been developed to determine viscosity in solutions or biological fluid. Herein, we report a distorted-BODIPY-based probe BV-1 for cellular viscosity, which is different from the conventional pure rotors. In BV-1, the internal steric hindrance between the meso-CHO group and the 1,7-dimethyl group forced the boron-dipyrrin framework to be distorted, which mainly caused nonradiative deactivation in low-viscosity environment. BV-1 gave high sensitivity (x=0.62) together with stringent selectivity to viscosity, thus enabling viscosity mapping in live cells. Significantly, the increase of cytoplasmic viscosity during apoptosis was observed by BV-1 in real time.