Release Time:2019-03-09  Hits:

Indexed by: Journal Article

Date of Publication: 2013-05-15

Journal: BIOORGANIC & MEDICINAL CHEMISTRY LETTERS

Included Journals: Scopus、PubMed、SCIE

Volume: 23

Issue: 10

Page Number: 2916-2919

ISSN: 0960-894X

Key Words: Fluorescence; Probe; Cu2+; Rhodamine; Cell imaging

Abstract: A novel rhodamine spirolactam derivative 3',6'-Bis(diethylamino)-2-(2-hydroxyethylamino) spiro[iso-indoline-1,9'-xanthen]-3-one (RO1) was synthesized, and characterized by high-resolution mass spectrometry (HRMS), X-ray crystallography, Infrared spectroscopy (IR), and H-1 NMR and C-13 NMR spectroscopy. RO1 exhibited highly sensitive and exclusively selective fluorescence response toward Cu2+ over other metal ions with a detection limit of 0.56 ppb in mixed aqueous solution. The fluorescence was pH-independent in the wide range pH 3.1-11.6. The turn-on fluorescence enhancement of the probe is based on Cu2+ induced ring-opening mechanism of the rhodamine spirolactam. Moreover, by means of fluorescence microscopy experiments, it was demonstrated that RO1 could monitor trace Cu2+ changes by live cell imaging. (C) 2013 Elsevier Ltd. All rights reserved.