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Indexed by:Journal Papers

Date of Publication:2019-12-01

Journal:DYES AND PIGMENTS

Included Journals:EI、SCIE

Volume:171

ISSN No.:0143-7208

Key Words:Two-photon; Carbazole derivative; Fluorescent probe; G-quadruplex DNA; Pyridostatin

Abstract:G-quadruplex (G4) sequences are considered to play important roles in gene regulation, therefore the development of selective and sensitive probes for G4 DNAs is important for studying the functions of G-rich gene sequences, as well as designing of novel and effective anticancer drugs. Herein, a carbazole derivative (CZ-BT) was synthesized and characterized by a simple process for G4 DNA detection. CZ-BT was preferentially bound with G4 DNA compared with other types of nucleic acids according to the fluorescence assays. The fluorescence intensity and fluorescence lifetime of CZ-BT significantly increased after the interaction with G4 DNA. Molecular docking calculation proved the pi-pi stacking binding mode between CZ-BT and G4 DNA. Furthermore, the specificity of CZ-BT on G4 DNA was well demonstrated with the contrast of pyridostatin (PDS, a classical G-quadruplex ligand) using two-photon confocal fluorescent imaging technique and cell cycle experiment in cells. We believe that this study would give some favorable factors on developing of highly effective fluorescent probes for G4 DNA applications.