Title of Paper:High-level expression of a soluble snake venom enzyme, gloshedobin, in E-coli in the presence of metal ions

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Date of Publication:2003-04-01

Journal:BIOTECHNOLOGY LETTERS

Included Journals:Scopus、SCIE、PubMed

Volume:25

Issue:8

Page Number:607-610

ISSN No.:0141-5492

Key Words:Escherichia coli; expression; gloshedobin; thrombin-like enzyme

Abstract:The mature gene of gloshedobin, a snake venom thrombin-like enzyme from the snake, Gloydius shedaoensis, was cloned and expressed in strain E. coli BL21(DE3). Having been induced by IPTG, the recombinant gloshedobin was in both soluble and insoluble forms. To avoid inclusion body formation, expression was optimized at 25degreesC. Furthermore, a 50% increase in solubilization of the target protein was obtained by adding 0.1 mM Mg2+ to the medium. The purified recombinant gloshedobin gave a 44 kDa band on SDS-PAGE gel.