Associate Professor
Supervisor of Master's Candidates
Main positions: Associate Professor in Biochemistry & Molecular Biology
Other Post: Vice Director, Panjin Institute of Industrial Technology (PIIT) of DUT
Title of Paper:Expression of gloshedobin, a thrombin-like enzyme from the venom of Gloydius shedaoensis, in Escherichia coli
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Date of Publication:2003-01-01
Journal:BIOTECHNOLOGY LETTERS
Included Journals:Scopus、SCIE、PubMed
Volume:25
Issue:2
Page Number:101-104
ISSN No.:0141-5492
Key Words:Escherichia coli; expression; gloshedobin; thrombin-like enzyme
Abstract:Gloshedobin, a thrombin-like enzyme from the venom of Gloydius shedaoensis, was expressed in Escherichia coli using expression vector pET-32a(+). The gene was expressed under T7 promotor with a fusion partner of Thx. Tag and a 6xHis. Tag at its 5' terminal. After induction by IPTG for 6 h, the recombinant enzyme was expressed in the cytoplasm. Expression at 25degreesC gave twice the amount of recombinant gloshedobin in cytoplasm than at 37degreesC.
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