论文类型：期刊论文
发表刊物：BIOTECHNOLOGY LETTERS
收录刊物：PubMed、SCIE、Scopus
卷号：25
期号：8
页面范围：607-610
ISSN号：0141-5492
关键字：Escherichia coli; expression; gloshedobin; thrombin-like enzyme
摘要：The mature gene of gloshedobin, a snake venom thrombin-like enzyme from the snake, Gloydius shedaoensis, was cloned and expressed in strain E. coli BL21(DE3). Having been induced by IPTG, the recombinant gloshedobin was in both soluble and insoluble forms. To avoid inclusion body formation, expression was optimized at 25degreesC. Furthermore, a 50% increase in solubilization of the target protein was obtained by adding 0.1 mM Mg2+ to the medium. The purified recombinant gloshedobin gave a 44 kDa band on SDS-PAGE gel.