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Indexed by:期刊论文

Date of Publication:2013-04-01

Journal:ASIAN JOURNAL OF CHEMISTRY

Included Journals:SCIE、Scopus

Volume:25

Issue:4

Page Number:2054-2058

ISSN No.:0970-7077

Key Words:Cytochrome B5; Quenching mechanism; Spectrosciopic techniques

Abstract:The interaction between Cu2+ and cytochrome b5 (cytb5) has been investigated by multi-spectroscopic techniques. The results show that Cu2+ could interact with cytochrome b5 and induce fluorescence of the latter to be quenched. The quenching mechanism is dominated by dynamic quenching. Synchronous fluorescence and circular dichroism spectra demonstrate that Cu2+ could also influence the microcircumatance of aromatic amino residues and secondary structure of the protein.