Release Time:2019-03-09  Hits:

Indexed by: Journal Article

Date of Publication: 2013-01-01

Journal: CHEMISTRY-A EUROPEAN JOURNAL

Included Journals: Scopus、PubMed、EI、SCIE

Volume: 19

Issue: 5

Page Number: 1548-1553

ISSN: 0947-6539

Key Words: cells; fluorescence; sensors; two-photon microscopy; viscosity

Abstract: In the thick of it: A carbazole-based cyanine derivative has been used as a ratiometric fluorescent sensor to detect mitochondrial viscosities over the range 1-950 cP within live cells and living tissues by using two-photon microscopy. Its success is based on the fast- and low-barrier rotation around its methine bond (see figure) and the huge increase of the fluorescence quantum yield (QY) from the loss of mobility in viscous media. Copyright © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.