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Indexed by:期刊论文

Date of Publication:2013-01-01

Journal:CHEMISTRY-A EUROPEAN JOURNAL

Included Journals:SCIE、EI、PubMed、Scopus

Volume:19

Issue:5

Page Number:1548-1553

ISSN No.:0947-6539

Key Words:cells; fluorescence; sensors; two-photon microscopy; viscosity

Abstract:In the thick of it: A carbazole-based cyanine derivative has been used as a ratiometric fluorescent sensor to detect mitochondrial viscosities over the range 1-950 cP within live cells and living tissues by using two-photon microscopy. Its success is based on the fast- and low-barrier rotation around its methine bond (see figure) and the huge increase of the fluorescence quantum yield (QY) from the loss of mobility in viscous media. Copyright © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.