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Indexed by:期刊论文

Date of Publication:2012-02-01

Journal:TOXICOLOGY IN VITRO

Included Journals:SCIE、PubMed、Scopus

Volume:26

Issue:1

Page Number:32-41

ISSN No.:0887-2333

Key Words:Single-walled carbon nanotube; Vitamin E; PC12 cells; ROS

Abstract:Single-walled carbon nanotubes (SWCNTs) are potential candidates in many biomedical applications. However, many reports demonstrated its potential toxicity to human and other biological systems. Our study has demonstrated that SWCNTs can induce apoptosis and oxidative damage on PC12 cells, an in vitro model of neuronal cells. In the present study, we for the first time investigated the neuroprotective effects of vitamin E (VE) on SWCNT-induced neurotoxicity in cultured PC12 cells. Vitamin E (0.01-2 mM) increased PC12 cells viability and significantly attenuated SWCNTs-induced apoptotic cell death in a time and dose-dependent manner, as demonstrated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, lactate dehydrogenase (LDH) release and morphological observation. The presence of VE inhibited the formation of reactive oxygen species (ROS), decreased the level of lipid peroxide, elevated the level of glutathione (GSH) and activities of superoxide dismutase (SOD), glutathione peroxidase (GPx), and catalase (CAT). Additionally, VE blocked the reduction in the mitochondrial membrane potential and the activation of caspase-3. VE prevented the down-regulation of Bcl-2 expression and up-regulation of Bax expression induced by SWCNTs in PC12 cells. In summary, VE might protect PC12 cells from the injury induced by SWCNTs through the down-regulation of oxidative stress and prevention of mitochondrial-mediated apoptosis. (C) 2011 Elsevier Ltd. All rights reserved.